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Cell culture is an invaluable tool for investigators in numerous fields.It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques. There are four basic requirements for successful cell culture.The first necessity is a well-established and properly equipped cell culture facility. The second requirement for successful cell culture is the practice of sterile technique. A third necessity for successful cell culture is appropriate, quality controlled reagents and supplies. The final necessity for successful cell culture is the knowledge and practice of the fundamental techniques involved in the growth of the cell type of interest. this book organised into 24 chapters.