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Protein expression: a practical approach describes in detail the expression of cloned DNA or RNA templates in all the major in vitro and in vivo systems, both prokaryotic and eukaryotic, as well as methods for monitoring expression. The in vivo systems include cultured mammalian cells (described comprehensively by Marlies Otter-Nilsson and Tommy Nilsson), yeast (by Mick Tuite and his colleagues), baculovirus (Bob Possee et al.), and Xenopus (Glenn Matthews). Expression in vivo in prokaryotes is covered by Ed Appelbaum and Allan Shatzman. On the in vitro side, the chapter by Mike Clemens and Ger Pruijn focuses on the purification of eukaryotic mRNA and its translation in cell-free extracts. The prokaryotic in vitro systems of note are those that offer coupled transcription-translation and hence these are the subject of the chapter by Boyd Hardesty's group. Finally, John Colyer's chapter provides essential techniques for monitoring protein expression. The overriding goals of Protein expression: a practical approach are to describe, in precise detail, tried and tested versions of key protocols for the active researcher, and to provide all the support required to make the techniques work optimally, including hints and tips for success, advice on potential pitfalls, and guidance on data interpretation.