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Embryonic Stem Cells Methods and Protocols is a part of Methods in Molecular Biology volume 185. Embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our understanding of cell lineages and differentiation. Embryonic Stem Cells: Methods and Protocols, a broadscaled work of 35 chapters containing step-by-step protocols suitable for use by both experienced investigators and novices in various ES cell technologies. In the first section of the volume, there are chapters with detailed protocols for ES cell isolation, maintenance, modulation of gene expression, and studies of ES cell cycle and apoptosis. Embryonic Stem Cells also includes chapters with protocols for the use of ES cells to generate diverse cell and tissue types, including blood, endothelium, adipocytes, skeletal muscle, cardiac muscle, neurons, osteoclasts, melanocytes, keratinocytes, and hair follicle cells. The second part of the volume contains a series of cutting edge techniques that have already been shown to have, or will soon have, tremendous utility with ES cells and their differentiated progeny. These chapters include the use of cDNA arrays in gene expression analysis, phage display antibody libraries to generate antibodies against very rare antigens, and phage display libraries to identify and characterize protein and protein interactions, to name a few.