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Physics and Chemistry Basis for Biotechnology is a volume in the "Focus on Biothechnology" series contains selected presentations from this meeting, A collection of experts has made serious efforts to present some of the latest developments in various scientific fields and to unveil prospective evolutions on the threshold of the new millenium. In reading the different chapters, it appears that more than ever significant advances in biotechnology very often depend on breakthroughs in the biotechnology itself (e.g. new instruments, production devices, detection methods), which - in turn - can be realized by implementing the appropriate physical and chemical principles into the new application. This ‘common’ pattern is illustrated in the different chapters. Some highly relevant, next generation scientific topics that are treated deal with de novo synthesis of materials for gene transfection, imaging contrast agents, radiotherapy, aroma measurements, psychrophilic environments, biomimetic materials, bioradicals, biosensors, and more. Some of the topics will give useful, up-to-date information on scientific aspects that may be either right in, or at the interface of their own field of research. Authors who contribute to this volume are: 1. Aleksey Nedoluzhko and Trevor Douglas (Biomimetic materials synthesis) 2. L . Henry Bryant, Jr . and Jeff W.M. Bulte (Chemical principles and biotechnology applications) 3. Sheila J . Sadeghi. Georgia E . Tsotsou, Michael Fairhead. Yergalem T . Meharenna and Gianfranco Gilardi (Rational design of P450 enzymes for biotechnology) 4. Elisabeth Csöregi, Szilveszter GÁspÁr, Mihaela Niculescu, Bo Mattiasson. Wolfgang Schuhmann (Amperometric enzyme-based biosensors for application in food and beverage industry) 5. Britta Lindholm-Sethson (Supported lipid membranes for reconstitution of membrane proteins) 6. P . Robberecht, M . Waelbroeck, and N . Moguilevsky (Functional structure of the secretin receptor) 7. D. Georlette, M. Bentahir, P. Claverie, T. Collins, S. D’amico, D. Delille. G . Feller, E . Gratia, A . Hoyoux, T . Lonhienne, M-A . Meuwis, L . Zecchinon and Ch . Gerday (Cold-adapted enzymes) 8. J.W.M. Bulte and L.H. Bryant Jr (Molecular and cellular magnetic resonance contrast agents) 9. Urs Häfeli (Radioactive microspheres for medical applications) 10. Wim Mondelaers and Philippe Lahorte (Physical. chemical and biological aspects) 11. Philippe Lahorte and Wim Mondelaers (Technologies and research ) 12. Saskia M . Van Ruth (Recent developments in isolation and characterisation)