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Due to their versatility and resolution, chromatographic separations of complex mixtures of biologicals are used for many purposes in academia and industry. This book introduces the reader to some of the recently proposed solutions. Capillary electrochromatography (CEC), for example, the latest and most promising branch of analytical chromatography, is still hindered from finding broader application by difficulties related to something as simple as the packing of a suitable column. The latest solutions for this but also the state of art of CEC in general are discussed in the chapter written by Frantisek Svec. The difficulty of combining speed, resolution and capacity when using the classical porous bead type stationary phases has even been called the “dilemma of protein chromatography”. Much progress has been made in this area by the advent of monolithic and related continuous stationary phases. The complex nature of many of the samples to be analyzed and separated in biochromatography often requires the use of some highly specific (“affinity”) ligands. Authors from industry join to report on their experience with chromatography as a continuous preparative process, which are : 1. Frantisek Svec (Capillary Electrochromatography:A Rapidly Emerging Separation Method) 2. Ales Strancar · Ales Podgornik · Milos Barut · Roman Necina (Short Monolithic Columns as Stationary Phases for Biochromatography) 3. Shaofeng Xie1 · Robert W. Allington · Jean M.J. Fréchet · Frantisek Svec (Porous Polymer Monoliths: An Alternative to Classical Beads) 4. Oliver Brüggemann (Molecularly Imprinted Materials – Receptors More Durable than Nature Can Provide) 5. Ales Podgornik · Tatiana B. Tennikova (Chromatographic Reactors Based on Biological Activity) 6. Sabine Imamoglu (Simulated Moving Bed Chromatography (SMB) for Application in Bioseparation)