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The first of this is the subset for biotechnologist, which contains selected procedures that provide essential technical information for this group of scientist. Cell and Tissue Culture: Laboratory procedures in biotechnology is organized into 6 chapters, which are: Chapter 1 - The cell: selection and standardization (Overview, Cell lines for Biotechnologist, Master and Working Cell Banks, Identity Testing - An Overview, DNA Fingerprinting, Detection of Mycoplasma, Mycoplasma detection Methods using PCR, Bacteria and Fungi, Elimination of Contamination. Capter 2 - Cell Qualification (Overview, Haemocytometer cell count and viability stdies, MTT Assay, Neutral Red (NR) Assay, LDH Assay, Miniaturized colorimetric methods for determining cell number) Chapter 3 - Culture Environment (Overview, serum-free systems, adaptation to serum-free culture, Amino acid metabolism, tissue culture surfaces, Plastic and glass tissue culture surfaces, Three-dimensional cell-culture systems) Chapter 4 - Biochemistry of cells in culture (overview, Quantitative analysis of cell growth, metabolism and product formation, Modeling, cell death in culture systems (kinetics of cell death), detoxification of cell cultures, oxigenation, mixing, mechanical protection). Chapter 5 - Culture Processes and scale-up (overview, roller bottle culture, spinner flask culture,Pilot-scale suspension culture of hybridomas-an overview, pilot-scale suspension culture of human hybridomas, chemost culture, growth of human diploid fibroblast for vaccine production multiplate culture, microcarries-basic techniques, porous microcarier and fixed-bed cultures, Control processes). Chapter 6 - Regulatory Issues (Regulatory aspects of cells utilized in biotechnological processes)