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Biophysics is an interdisciplinary subject, and its treatment varies with the background of both the author and the reader. For example, some aspects of theoretical biophysics are indistinguishable from pure mathematics. On the other hand, modern biophysics has made profound contributions to subjects normally considered a part of classical biology, such as evolution. Many of the explanations may therefore appear too elementary and too verbose to sophisticated readers. Chapter 1 gives an introduction to laws of physics and chemistry and is designed to reinforce and enhance slightly the school-level understanding of the subject, which students with a background of purely biological science would have. This chapter covers quantum mechanics, molecular orbital theory, essentials of weak and strong molecular interactions, stereochemistry, fundamentals of thermodynamics and radiation biophysics. Chapters 2 and 3 deal with separation techniques and physico-chemical techniques used to study biomolecular structure. Strictly speaking this would be counted under physical biochemistry, rather than purely biophysics. The coverage is not exhaustive and is intended to give an overview of various techniques available to unravel the properties of biomolecules. Chapters 4 to 9 describe some of the spectroscopy, microscopy, diffraction and computational techniques. These techniques are so powerful that every biophysicist must be armed with this knowledge so that it can be put to use when the need arises. These chapters aim at providing an understanding of the basic principles involved rather than thorough knowledge of the theory and practice of the techniques. The descriptions would enable most biologists to obtain an understanding of the relative merits and demerits of each technique, in order that the biochemical results, which one may come across in the general literature, become intelligible. X-ray crystallography and NMR spectroscopy have been dealt with in greater detail owing to their prominence in determining biomolecular structures. Chapter 10 obtained results from the application of the techniques of structural biology are reviewed. This chapter is illustrated by the largest number of diagrams. The later chapters try and fill the gaps between biophysicists and biochemists. The treatment of Energy Pathways in Chapter 11 involves a lot of physical chemistry. Biomechanics in Chapter 12 and Neurophysics in Chapter 13 involve a lot of physiology. Though the advancements in the application of physical principles in these fields have been phenomenal they are probably too advanced and too detailed for the intended audience of this book.