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What actually is biophysics? Biophysics is neither "physics for biologists nor physical methods applied to biology, but a modern field of science leading to new approaches for our understanding of biological functions. The philosophy of this book is that biophysics is not a simple collection of physical approaches to biology, but a defined discipline eith its own network of ideas and approaches, spanning all hierarchical levels of biological organizations. the paradigm of a holistic view of biological functions, where the biological system is not simply the sum of its molecular components but is rather their functional integration, seems to be the main concepts of biophysics. This book is written primarily for biologists and biophysicists with a background in biology. Therefore, some basic knowledge of biology required, but less knowledge of physics and mathematics is needed. Biophysics 4th ed is organized into 5 part: Nature and subject of biophysics, Molecular structure of biological systems, Energetics and dynamics of biological systems, Physical factors of the Environment and The Kinetics of Biological Systems. At least, this book will help to answer the question: what is biophysics? It provide a solid introduction to biophysics. for further reading, books and reviews are recommended at the end of each chapter. The extensive index at the end of the book ensures as easy orientation and will enable this book to be used as a reference work.